Development of an HTRF method to determine the stoichiometry of subunits...
MAb Anti cmyc-XL665 HTRF®
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No-wash
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Ease-of-use
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High affinity
Overview
MAb Anti cmyc-XL665 is an IgG1 raised against a synthetic peptide corresponding to the 408-439 sequence of human c-myc protein labeled with XL. It recognizes the EQKLISEEDL motif and is specific for human c-myc, although a slight cross-reaction has been observed with murine c-myc at high antibody concentration.
This reagent can be used in both biochemical and cellular formats to study a wide variety of interactions: protein/protein, protein/peptide, protein/DNA, receptor/ligand.
HTRF can detect a broad range of affinity constants ranging from picomolar to low millimolar.
Benefits
- LARGE COMPLEXES DETECTION
- BATCH-TO-BATCH REPRODUCIBILITY
- LOW TO HIGH KD DETECTION
Assay principle
Assay protocol
Build HTRF interaction assays for your specific application
How do the number of tests relate to active moiety?
Motif/Species | Antibody | Active Moiety/5,000 tests | Species and Subtype | Specificity |
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cMYC | MAb 9E10 | 5-10 µg | Mouse IgG1 | EQKLISEEDL peptide |
Recommended quantities of Cryptate and XL665 conjugates
Applications of HTRF and Tag-lite-lite Assays for HTP Antibody Screening
In collaboration with Bristol Myers Squibb - Scientific Presentations
Academia/Industry Cross-Fertilization through Translational Research
In collaboration with Scripps - Scientific Presentations
HTRF-powered detection of ubiquitin-like protein (UBL) signaling and interactions
In collaboration with Sanford/Burnham - Scientific Presentations
The use of HTRF in biologics discovery
In collaboration with MedImmune - Scientific Presentations
HTRF: One technology, many uses in a pre-clinical laboratory setting
Benefits and considerations of HTRF - Scientific Presentations
Exploiting HTRF for novel drug classes: Stabilizing 14-3-3 protein-protein interactions
In collaboration with Lead Discovery Center GmbH - Scientific Presentations
HTRF a versatil approach for 7TM drug discovery
In collaboration with MRC - Scientific Presentations
HTRF protein-protein interaction reagents
Benefit from unlimited flexibility for your assay development - Flyers
Addressing the interactome with protein-protein assays
Cover all PPIs with one approach - Brochures
HTRF -A Beneficial Tool for Lead Optimization of Biotherapeutics
In collaboration with Boehringer Ingelheim - Posters
The ultimate guide to PPI assays with HTRF
Complete guide - Guides
Guidelines for optimizing protein-protein interaction assays using HTRF PPI reagents
Every interaction is within range - Technical Notes
Product Insert Anti cmyc-XL / 61MYCXLF-61MYCXLA-61MYCXLB
61MYCXLF-61MYCXLA-61MYCXLB - Product Insert
HTRF PPI your dream assay served on a plate
Sandwiches aren't just for eating - Infographics
Best practices for pharmacological characterization of PPI inhibitors
Easy pharmacological characterization of PPI modulators. - Technical Notes
HTRF assays handle low- to high affinity protein-protein interactions
Deciphering low- and high affinity interactions - Application Notes
Nuclear receptor ligand identification with HTRF
Monitoring nuclear receptor binding with HTRF assays - Application Notes
HTRF addresses large protein-protein interaction complexes
Challenge large complexes with HTRF assays - Application Notes
A brief history of Protein-Protein Interactions
How well do you know PPI? - Infographics
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Virology research solutions using HTRF Protein-Protein Interaction assays
See how peer researchers challenge the viral life cycle with PPI assays - Application Notes
Advance your CAR-T cell research - Flyers
Safety Data Sheet Anti cmyc-XL / 61MYCXLA
61MYCXLA - Safety Data Sheet
Safety Data Sheet (DEU) Anti cmyc-XL / 61MYCXLA
61MYCXLA - Safety Data Sheet
Safety Data Sheet (ELL) Anti cmyc-XL / 61MYCXLA
61MYCXLA - Safety Data Sheet
Safety Data Sheet (FRA-FR) Anti cmyc-XL / 61MYCXLA
61MYCXLA - Safety Data Sheet
Safety Data Sheet (ITA) Anti cmyc-XL / 61MYCXLA
61MYCXLA - Safety Data Sheet
Safety Data Sheet (SPA) Anti cmyc-XL / 61MYCXLA
61MYCXLA - Safety Data Sheet
Safety Data Sheet (ENG-GB) Anti cmyc-XL / 61MYCXLA
61MYCXLA - Safety Data Sheet
Safety Data Sheet (ENG-US) Anti cmyc-XL / 61MYCXLA
61MYCXLA - Safety Data Sheet
Batch Information Anti cmyc-XL / 20240111
61MYCXLA Batch 46A - Batch Information
Safety Data Sheet Anti cmyc-XL / 61MYCXLB
61MYCXLB - Safety Data Sheet
Safety Data Sheet (DEU) Anti cmyc-XL / 61MYCXLB
61MYCXLB - Safety Data Sheet
Safety Data Sheet (ELL) Anti cmyc-XL / 61MYCXLB
61MYCXLB - Safety Data Sheet
Safety Data Sheet (FRA-FR) Anti cmyc-XL / 61MYCXLB
61MYCXLB - Safety Data Sheet
Safety Data Sheet (ITA) Anti cmyc-XL / 61MYCXLB
61MYCXLB - Safety Data Sheet
Safety Data Sheet (SPA) Anti cmyc-XL / 61MYCXLB
61MYCXLB - Safety Data Sheet
Safety Data Sheet (ENG-GB) Anti cmyc-XL / 61MYCXLB
61MYCXLB - Safety Data Sheet
Safety Data Sheet (ENG-US) Anti cmyc-XL / 61MYCXLB
61MYCXLB - Safety Data Sheet
Batch Information Anti cmyc-XL / 20240111
61MYCXLB Batch 46A - Batch Information
Safety Data Sheet (DEU) Anti cmyc-XL / 61MYCXLF
61MYCXLF - Safety Data Sheet
Safety Data Sheet (ELL) Anti cmyc-XL / 61MYCXLF
61MYCXLF - Safety Data Sheet
Safety Data Sheet (FRA-FR) Anti cmyc-XL / 61MYCXLF
61MYCXLF - Safety Data Sheet
Safety Data Sheet (ITA) Anti cmyc-XL / 61MYCXLF
61MYCXLF - Safety Data Sheet
Safety Data Sheet (SPA) Anti cmyc-XL / 61MYCXLF
61MYCXLF - Safety Data Sheet
Safety Data Sheet (ENG-GB) Anti cmyc-XL / 61MYCXLF
61MYCXLF - Safety Data Sheet
Safety Data Sheet (ENG-US) Anti cmyc-XL / 61MYCXLF
61MYCXLF - Safety Data Sheet
Batch Information Anti cmyc-XL / 20230427
61MYCXLF Batch 44B - Batch Information
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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