CD16a (FcgRIIIa, V158) cellular binding kit
CD16a (FcgRIIIa, V158) cellular binding assay for ADCC prediction
CD16a (FcgRIIIa, F158) cellular binding kit HTRF®
The CD16a cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD16a receptor on live cells.
See more
-
Cellular binding assay
-
No-wash
-
All inclusive kit
Overview
The CD16a (FcgRIIIa, F158) cellular binding assay is a simple and robust test to monitor binding of the IgG Fc region to the human CD16a receptor in live cells and to select the most potent antibody for use in Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC). The assay can also be used to assess the degree of antibody afucosylation. Two separate CD16a assays are available to specifically target the V158 or F158 variants. These assays, based on our innovative Tag-lite technology, can easily be automated to create a high-throughput solution for predicting ADCC.
Benefits
- CORRELATED WITH ADCC READOUTS
- FAST ANTIBODY SELECTION
- AFUCOSYLATION LEVEL ASSESSMENT
Assay principle
The CD16a receptor cellular binding assay is a competitive assay involving HEK293 cells expressing a specific CD16a variant conjugated to Lumi4-terbium Cryptate and human IgG1 labelled with d2. The unlabelled antibody or Fc tagged drug competes with the d2 labelled IgG1 for binding to the receptor, resulting in a decrease of FRET signal.
Assay protocol
The CD16a receptor cellular binding assays (FcgIIIa V158 or F158) present only 1 incubation step after reagents distribution. Lumi4-terbium Cryptate pre-labelled cells are dispensed, followed by the addition of IgG1 labelled with d2 and the sample. After 2h incubation at RT, the signal can be read.
Specificity for IgG subclasses
Increasing concentrations of unlabeled IgGs were incubated in presence of a constant concentration of d2-labeled IgG1. The plate was then read on a PHERAstar microplate reader (BMG Labtech). As previously described, it was confirmed that the affinity of IgG1 for CD16a is better than that of IgG3 and considerably higher than IgG2 and IgG4 (Bruhns et al., Blood 2009).
A-fucosylation assessment
To evaluate the impact of IgG a-fucosylation on CD16a affinity, the same IgG with various degrees of fucosylation was tested following the previously described protocol. IgG affinity for CD16a and degree of a-fucosylation were very well correlated. These results confirm that a substantial gain of immunoglobulin affinity can be reached when the level of a-fucosylation is higher than 50%. The CD16a assay can therefore be used for the prediction of a-fucosylation degree and ADCC potential.
IgG core a-fucosylation and its impact on FcgammaRIIIa binding
In collaboration with Roche - Scientific Presentations
Tag-lite® services to evaluate your Biosimilar or Biobetter samples
profile and study your Fcg receptor - Flyers
In collaboration with BioTek - Posters
TagLite technology: adding a new level to drug discovery analysis
In collaboration with Roche Glycart - Posters
A cellular binding platform to measure human IgG affinity for Fc gamma receptors (FcyRs)
Characterizing therapeutic antibodies - Posters
Therapeutic development with robust cellular binding assays
Tag-lite® CD16a cellular binding assays - Flyers
Product Insert CD16a (F158) Kit / 6FR3APAG-6FR3APAE-6FR3APAF
6FR3APAG-6FR3APAE-6FR3APAF - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Overcoming challenges for treating solid tumors
Insight on experiments carried out by peer researchers focusing on solid tumors - Application Notes
Safety Data Sheet (DEU) CD16a (F158) Kit / 6FR3APAE
6FR3APAE - Safety Data Sheet
Safety Data Sheet (ELL) CD16a (F158) Kit / 6FR3APAE
6FR3APAE - Safety Data Sheet
Safety Data Sheet (FRA-FR) CD16a (F158) Kit / 6FR3APAE
6FR3APAE - Safety Data Sheet
Safety Data Sheet (ITA) CD16a (F158) Kit / 6FR3APAE
6FR3APAE - Safety Data Sheet
Safety Data Sheet (SPA) CD16a (F158) Kit / 6FR3APAE
6FR3APAE - Safety Data Sheet
Safety Data Sheet (ENG-GB) CD16a (F158) Kit / 6FR3APAE
6FR3APAE - Safety Data Sheet
Safety Data Sheet (ENG-US) CD16a (F158) Kit / 6FR3APAE
6FR3APAE - Safety Data Sheet
Safety Data Sheet (DEU) CD16a (F158) Kit / 6FR3APAF
6FR3APAF - Safety Data Sheet
Safety Data Sheet (ELL) CD16a (F158) Kit / 6FR3APAF
6FR3APAF - Safety Data Sheet
Safety Data Sheet (FRA-FR) CD16a (F158) Kit / 6FR3APAF
6FR3APAF - Safety Data Sheet
Safety Data Sheet (ITA) CD16a (F158) Kit / 6FR3APAF
6FR3APAF - Safety Data Sheet
Safety Data Sheet (SPA) CD16a (F158) Kit / 6FR3APAF
6FR3APAF - Safety Data Sheet
Safety Data Sheet (ENG-GB) CD16a (F158) Kit / 6FR3APAF
6FR3APAF - Safety Data Sheet
Safety Data Sheet (ENG-US) CD16a (F158) Kit / 6FR3APAF
6FR3APAF - Safety Data Sheet
Safety Data Sheet (DEU) CD16a (F158) Kit / 6FR3APAG
6FR3APAG - Safety Data Sheet
Safety Data Sheet (ELL) CD16a (F158) Kit / 6FR3APAG
6FR3APAG - Safety Data Sheet
Safety Data Sheet (FRA-FR) CD16a (F158) Kit / 6FR3APAG
6FR3APAG - Safety Data Sheet
Safety Data Sheet (ITA) CD16a (F158) Kit / 6FR3APAG
6FR3APAG - Safety Data Sheet
Safety Data Sheet (SPA) CD16a (F158) Kit / 6FR3APAG
6FR3APAG - Safety Data Sheet
Safety Data Sheet (ENG-GB) CD16a (F158) Kit / 6FR3APAG
6FR3APAG - Safety Data Sheet
Safety Data Sheet (ENG-US) CD16a (F158) Kit / 6FR3APAG
6FR3APAG - Safety Data Sheet
Safety Data Sheet (DEU) CD16a (F158) Kit / C6FR3APAE
C6FR3APAE - Safety Data Sheet
Safety Data Sheet (ELL) CD16a (F158) Kit / C6FR3APAE
C6FR3APAE - Safety Data Sheet
Safety Data Sheet (FRA-FR) CD16a (F158) Kit / C6FR3APAE
C6FR3APAE - Safety Data Sheet
Safety Data Sheet (ITA) CD16a (F158) Kit / C6FR3APAE
C6FR3APAE - Safety Data Sheet
Safety Data Sheet (SPA) CD16a (F158) Kit / C6FR3APAE
C6FR3APAE - Safety Data Sheet
Safety Data Sheet (ENG-GB) CD16a (F158) Kit / C6FR3APAE
C6FR3APAE - Safety Data Sheet
Safety Data Sheet (ENG-US) CD16a (F158) Kit / C6FR3APAE
C6FR3APAE - Safety Data Sheet
Safety Data Sheet (DEU) CD16a (F158) Kit / C6FR3APAF
C6FR3APAF - Safety Data Sheet
Safety Data Sheet (ELL) CD16a (F158) Kit / C6FR3APAF
C6FR3APAF - Safety Data Sheet
Safety Data Sheet (FRA-FR) CD16a (F158) Kit / C6FR3APAF
C6FR3APAF - Safety Data Sheet
Safety Data Sheet (ITA) CD16a (F158) Kit / C6FR3APAF
C6FR3APAF - Safety Data Sheet
Safety Data Sheet (SPA) CD16a (F158) Kit / C6FR3APAF
C6FR3APAF - Safety Data Sheet
Safety Data Sheet (ENG-GB) CD16a (F158) Kit / C6FR3APAF
C6FR3APAF - Safety Data Sheet
Safety Data Sheet (ENG-US) CD16a (F158) Kit / C6FR3APAF
C6FR3APAF - Safety Data Sheet
Safety Data Sheet (DEU) CD16a (F158) Kit / C6FR3APAG
C6FR3APAG - Safety Data Sheet
Safety Data Sheet (ELL) CD16a (F158) Kit / C6FR3APAG
C6FR3APAG - Safety Data Sheet
Safety Data Sheet (FRA-FR) CD16a (F158) Kit / C6FR3APAG
C6FR3APAG - Safety Data Sheet
Safety Data Sheet (ITA) CD16a (F158) Kit / C6FR3APAG
C6FR3APAG - Safety Data Sheet
Safety Data Sheet (SPA) CD16a (F158) Kit / C6FR3APAG
C6FR3APAG - Safety Data Sheet
Safety Data Sheet (ENG-GB) CD16a (F158) Kit / C6FR3APAG
C6FR3APAG - Safety Data Sheet
Safety Data Sheet (ENG-US) CD16a (F158) Kit / C6FR3APAG
C6FR3APAG - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
Let's find your reader
You might be interested in