Anti histone H3K9 Me2-EuK mAb conjugate
Eu-cryptate conjugate for H3K9Me2 epigenetic mark detection
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Cisbio offers a line of ready to use HTRF toolbox reagents for the construction of Histone H3 methyltransferase (HMT), demethylase (HDM) and deacetylase (HDAC) activity assays. This monoclonal antibody Eu-cryptate conjugate is specific for the histone H3K9Me0 methylation mark. It can be used for the development of biochemical assays with the highest levels of specificity and sensitivity, for the screening and study of both DMTs such as JMJD1A, and HDACs 1, 3-8, as well as SIRT1-3 and 6.
Methylation level | K4 | K9 | K27 | K36 |
---|---|---|---|---|
Me0 | V | V | ||
Me1 | V | V | V | V |
Me2 | V | V | V | V |
Me3 | V | V |
PeptideMAb | H3K4 | H3K9 | H3K27 | H3K36 | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
me0 | me1 | me2 | me3 | me0 | me1 | me2 | me1 | me2 | me3 | me1 | me2 | |
H3(1-21) | 100% | 1.3% | 0% | 0% | 100% | 0.7% | 0% | - | - | 0% | - | - |
H3(21-44) | 0% | - | - | 0% | - | - | 0% | 0% | 0% | 0% | 4.4% | 0% |
H3(1-21) K4Ac | 0% | - | - | - | - | - | - | - | - | - | - | - |
H3(1-21) K9Ac | - | - | - | - | 0.1% | - | - | - | - | - | - | - |
H3(1-21) K4me1 | 0% | 100% | 0.6% | 0.5% | - | - | 0% | - | - | 0% | - | - |
H3(1-21) K4me2 | 0% | 15% | 100% | 7.4% | - | - | 0% | - | - | 0% | - | - |
H3(1-21) K4me3 | 0% | 0.4% | 0% | 100% | - | - | 0% | - | - | 0% | - | - |
H3(1-21) K9me1 | - | - | - | 0% | 0.4% | 100% | 0.1% | - | - | 0% | - | - |
H3(1-21) K9me2 | - | - | - | 0% | 0.3% | 0% | 100% | - | - | 0% | - | - |
H3(1-21) K9me3 | - | - | - | 0.2% | 0.1% | 0% | 0.1% | - | - | 0.2% | - | - |
H3(21-44) K27me1 | - | - | - | 0% | - | - | 0.1% | 100% | 1.5% | 0.2% | - | 0% |
H3(21-44) K27me2 | - | - | - | 0.2% | - | - | 0% | 1.4% | 100% | 3.3% | - | 0.1% |
H3(21-44) K27me3 | - | - | - | 0% | - | - | 0.1% | 0% | 0% | 100% | - | 0% |
H3(21-44) K36me1 | - | - | - | 0% | - | - | 0.1% | - | - | 0% | 100% | 0.7% |
H3(21-44) K36me2 | - | - | - | 0% | - | - | 0.1% | - | - | 0.1% | 0.60% | 100% |
H3(21-44) K36me3 | - | - | - | 0.1% | - | - | 0.1% | - | - | 0.4% | 0% | 0% |
Enzyme | Type | Methylation level | Biotinylated substrate | Eu3+-Cryptate MAb | Application note | |
---|---|---|---|---|---|---|
JMJD1A | Demethylase | K9 | me2 →me0 | H3(1-21)K9me2 | H3K9me0 | |
G9a | Methyl transferase | K9 | me0 →me2 | H3(1-21)K9me0 | H3K9me2 | |
G9a | Methyl transferase | K9 | me1 →me2 | H3(1-21)K9me1 | H3K9me2 | |
JMJD2C | Demethylase | K9 | me3 →me2 | H3(1-21)K9me3 | H3K9me2 | |
HDAC1 | Deacetylase | K9 | Ac →unmodified | H3(1-21)K9Ac | H3K9me0 | |
HDAC2 | Deacetylase | K9 | Ac →unmodified | H3(1-21)K9Ac | H3K9me0 | |
HDAC3 | Deacetylase | K9 | Ac →unmodified | H3(1-21)K9Ac | H3K9me0 | |
HDAC6 | Deacetylase | K9 | Ac →unmodified | H3(1-21)K9Ac | H3K9me0 | |
HDAC8 | Deacetylase | K9 | Ac →unmodified | H3(1-21)K9Ac | H3K9me0 | |
SIRT1 | Deacetylase | K9 | Ac →unmodified | H3(1-21)K9Ac | H3K9me0 | |
SIRT2 | Deacetylase | K9 | Ac →unmodified | H3(1-21)K9Ac | H3K9me0 | |
SIRT3 | Deacetylase | K9 | Ac →unmodified | H3(1-21)K9Ac | H3K9me0 | |
SIRT6 | Deacetylase | K9 | Ac →unmodified | H3(1-21)K9Ac | H3K9me0 |
HTRF JMJD1A Histone H3K9 di-demethylation assay me2-->me0
Assay protocol and validations - Technical Notes
HTRF HDAC1 Histone H3K9 deacetylation assay
Assay protocol and validations - Technical Notes
HTRF HDAC2 Histone H3K4 deacetylation assay
Assay protocol and validations - Technical Notes
HTRF HDAC2 Histone H3K9 deacetylation assay
Assay protocol and validations - Technical Notes
HTRF HDAC3/NCOR2 Histone H3K9 deacetylation assay
Assay protocol and validations - Technical Notes
HTRF HDAC6 Histone H3K9 deacetylation assay
Assay protocol and validations - Technical Notes
HTRF HDAC8 Histone H3K9 deacetylation assay
Assay protocol and validations - Technical Notes
HTRF SIRT1 Histone H3K9 deacetylation assay
Assay protocol and validations - Technical Notes
HTRF SIRT2 Histone H3K9 deacetylation assay
Assay protocol and validations - Technical Notes
HTRF SIRT3 Histone H3K9 deacetylation assay
Assay protocol and validations - Technical Notes
HTRF SIRT6 Histone H3K9 deacetylation assay
Assay protocol and validations - Technical Notes
Histone Demethylase JARID1A and JARID1C Enzymatic applications with HTRF® Technology
High-Throughput and Homogeneous assays - Posters
Product Insert Bioch. H3K9 Me0 / 61KB0KAD-61KB0KAE
61KB0KAD-61KB0KAE - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Batch Information Bioch. H3K9 Me0 / 20231119
61KB0KAD Batch 01 - Batch Information
Safety Data Sheet Bioch. H3K9 Me0 / 61KB0KAD
61KB0KAD - Safety Data Sheet
Safety Data Sheet (DEU) Bioch. H3K9 Me0 / 61KB0KAD
61KB0KAD - Safety Data Sheet
Safety Data Sheet (ELL) Bioch. H3K9 Me0 / 61KB0KAD
61KB0KAD - Safety Data Sheet
Safety Data Sheet (FRA-FR) Bioch. H3K9 Me0 / 61KB0KAD
61KB0KAD - Safety Data Sheet
Safety Data Sheet (ITA) Bioch. H3K9 Me0 / 61KB0KAD
61KB0KAD - Safety Data Sheet
Safety Data Sheet (SPA) Bioch. H3K9 Me0 / 61KB0KAD
61KB0KAD - Safety Data Sheet
Safety Data Sheet (ENG-GB) Bioch. H3K9 Me0 / 61KB0KAD
61KB0KAD - Safety Data Sheet
Safety Data Sheet (ENG-US) Bioch. H3K9 Me0 / 61KB0KAD
61KB0KAD - Safety Data Sheet
Batch Information Bioch. H3K9 Me0 / 20300104
61KB0KAD Batch 02A - Batch Information
Batch Information Bioch. H3K9 Me0 / 20320405
61KB0KAD Batch 04A - Batch Information
Batch Information Bioch. H3K9 Me0 / 20320509
61KB0KAD Batch 05A - Batch Information
Batch Information Bioch. H3K9 Me0 / 20320509
61KB0KAD Batch 05RA - Batch Information
Safety Data Sheet Bioch. H3K9 Me0 / 61KB0KAE
61KB0KAE - Safety Data Sheet
Batch Information Bioch. H3K9 Me0 / 20231119
61KB0KAE Batch 01B - Batch Information
Safety Data Sheet (DEU) Bioch. H3K9 Me0 / 61KB0KAE
61KB0KAE - Safety Data Sheet
Safety Data Sheet (ELL) Bioch. H3K9 Me0 / 61KB0KAE
61KB0KAE - Safety Data Sheet
Safety Data Sheet (FRA-FR) Bioch. H3K9 Me0 / 61KB0KAE
61KB0KAE - Safety Data Sheet
Safety Data Sheet (ITA) Bioch. H3K9 Me0 / 61KB0KAE
61KB0KAE - Safety Data Sheet
Safety Data Sheet (SPA) Bioch. H3K9 Me0 / 61KB0KAE
61KB0KAE - Safety Data Sheet
Safety Data Sheet (ENG-GB) Bioch. H3K9 Me0 / 61KB0KAE
61KB0KAE - Safety Data Sheet
Safety Data Sheet (ENG-US) Bioch. H3K9 Me0 / 61KB0KAE
61KB0KAE - Safety Data Sheet
Batch Information Bioch. H3K9 Me0 / 20240406
61KB0KAE Batch 02A - Batch Information
Batch Information Bioch. H3K9 Me0 / 20320405
61KB0KAE Batch 04A - Batch Information
Batch Information Bioch. H3K9 Me0 / 20231119
61KB0KAE Batch 01 - Batch Information
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